Nonlinear DNA sign amplification with an enzyme-free isothermal self-assembly course of is uniquely helpful in nanotechnology and nanomedicine. Nevertheless, progress on this course is hampered by the dearth of efficient design fashions of leak-resistant DNA constructing blocks. Right here, we suggest two conceptual fashions of clever and strong DNA robots to carry out a leakless nonlinear sign amplification in response to a set off. Two conceptual fashions are based mostly on super-hairpin nanostructures, that are designed by innovating novel rules in methodology and codifying them into embedded packages. The dynamical and thermodynamical analyses reveal the crucial components and leak-resistant mechanisms of the designed fashions, and the leak-resistant behaviors of the clever DNA robots and morphologies of swarming into nonlinear amplification are individually verified. The functions of the designed fashions are additionally illustrated in particular sign amplification and focused payload enrichment through integration with an aptamer, a fluorescent molecule and surface-enhanced Raman spectroscopy. This work has the potential to function design tips of clever and strong DNA robots and leakless nonlinear DNA amplification, and in addition because the design blueprint of cargo supply robots with the efficiency of swarming into nonlinear amplification in response to a goal robotically, facilitating their future functions in biosensing, bioimaging and biomedicine.
