With a purpose to enhance the retention skill of anthraquinones and flavonoids on excessive efficiency liquid chromatography (HPLC) column and enhance chromatographic efficiency, multi-walled carbon nanotubes (MWCNTs) had been sure to the floor of silica spheres through amide bonding utilizing a chemical synthesis methodology. Three hybrid fillers had been ready by various the quantity of MWCNTs added, and the stationary phases had been characterised utilizing scanning electron microscopy (SEM), transmission electron microscopy (TEM) and Fourier remodel infrared spectroscopy (FTIR). The profitable preparation of the combined stationary phases was demonstrated by evaluating the adjustments within the floor of silica spheres earlier than and after bonding and the adjustments within the absorption peaks of particular practical teams. The combined stationary phases had been stuffed within the empty column of the HPLC. The outcomes of this examine point out that the columns may be ready by CNTs@SiO2 to enhance or broaden the chromatographic efficiency of the stationary part and acquire higher separation of the compounds. This thesis supplies an concept for bettering chromatographic efficiency with a view to taking part in a greater position within the software of compound separation.